EFFECT OF ALCOHOL ON TRYPSIN DIGESTION 



25 



digestion was found by precipitation with tannic acid and subsequent esti- 

 mation of the nitrogen in the filtrate. Controls showed that the sodium car- 

 bonate alone had no digestive action whatever either on fibrin or caseinogen. 

 The following are typical results showing the effect of dilute alcohol on the 

 digestion of fibrin and caseinogen by trypsin. 



c.c. trypsin, 20 c.c. 10 % alcohol, 20 c.c. 1 % NajCOs 

 c.c. trypsin, 20 c.c. water, 20 c.c. 1 % NajCOg 



4 g. fibrin added. Digestion 3 hours, 

 c.c. trypsin, 20 c.c. 10 % alcohol, 20 c.c. caseinogen 

 c.c. trypsin, 20 c.c. water, 20 c.c. caseinogen 



Digestion 1 hour. 



c.c. trypsin, 20 c.c. 12 % alcohol, 

 c.c. trypsin, 20 c.c. water, 

 g. fibrin. Digestion 2*75 hours, 

 c.c. trypsin, 20 c.c. 12 % alcohol, 

 c.c. trypsin, 20 c.c. water. 

 Digestion 1-25 hours. 



c.c. trypsin, 20 c.c. 10 % alcohol, 

 c.c. trypsin, 20 c.c. water, 

 3 g. fibrin. Digestion 2 hours, 

 c.c. trypsin, 20 c.c. 10 % alcohol, 

 c.c. trypsin, 20 c.c. water. 

 Digestion 1-25 hours. 



c.c. trypsin, 20 c.c. 10 % alcohol, 

 c.c. trypsin, 20 c.c. water, 

 5 g. fibrin. Digestion 2-25 hours, 

 c.c. trypsin, 20 c.c. 10 % alcohol, 

 c.c. trypsin, 20 c.c. water. 

 Digestion 1 hour. 



c.c. trypsin, 20 c.c. 8 % alcohol, 

 c.c. trypsin, 20 c.c. water, 

 g. fibrin. Digestion 3 hours, 

 c.c. trypsin, 20 c.c. 8 % alcohol, 

 c.c. trypsin, 20 c.c. water. 

 Digestion 1'25 hours. 



c.c. trypsin, 20 c.c. 8 % alcohol, 

 c.c. trypsin, 20 c.c. water, 

 g. fibrin. Digestion 3 hours, 

 c.c. trypsin, 20 c.c. 8 % alcohol, 

 c.c. trypsin, 20 c.c. water. 

 Digestion 1 hour. 



20 c.c. 1 % Na^COg 

 20 c.c. 1 % NaaCO;. 



20 c.c. caseinogen 

 20 c.c. caseinogen 



20 c.c. 1 % Na^COa 

 20 c.c. 1 % NajCOa 



20 c.c. caseinogen 

 20 c.c. caseinogen 



20 c.c. 1 % NaaCOg 

 20 c.c. 1 % Na.^C03 



20 c.c. caseinogen 

 20 c.c. caseinogen 



20 c.c. 1 % NajCOg 

 20 c.c. 1 % NagCOg 



20 c.c. caseinogen 

 20 c.c. caseinogen 



20 c.c. 1 % NaaCOg 

 20 c.c. 1 % Na^COs 



20 c.c. caseinogen 

 20 c.c. caseinogen 



c.c. trypsin, 20 c.c. 6 % alcohol, 20 c.c. 1 % NagCOa 



c.c. trypsin, 20 c.c. water, 20 c.c. 1 % NajCOs 

 g. fibrin. Digestion 3 hours. 



c.c. trypsin, 20 c.c. 6 % alcohol, 20 c.c. caseinogen 



c.c. trypsin, 20 c.c. water, 20 c.c. caseinogen 

 Digestion 1 hour. 



(221) 



Digestion in cc. 

 of N/IO nitrogen 



4-6 

 171 



23-8 

 23-6 



4-6 

 13-8 



241 

 24-3 



3-8 

 141 



27-9 

 27-0 



8-1 

 23-6 



300 

 29-3 



51 

 121 



30-6 

 30-6 



4-9 

 121 



27-7 

 28-0 



11-0 



20-3 



22-2 

 220 



