26 



E. S. EDIE 



1 c.c. trypsin, 20 c.c. 6 % alcohol, 

 1 c.c. trypsin, 20 c.c. water, 

 1 g. fibrin. Digestion 3 hours. 

 1 c.c. trypsin, 20 c.c. 6 % alcohol, 

 1 c.c. trypsin, 20 c.c. water. 

 Digestion 1 hour. 



5 c.c. trypsin, 20 c.c. 16 % alc«hol, 



6 c.c. trypsin, 20 c.c. water, 

 1 g. fibrin. Digestion 2 hours. 

 5 c.c. trypsin, 20 c.c. 16 % alcohol, 

 5 c.c. trypsin, 20 c.c. water. 

 Digestion 1 hour. 



5 c.c. trypsin, 20 c.c. 13 % alcohol, 

 5 c.c. trypsin, 20 c.c. water, 

 1-3 g. fibrin. Digestion 3 hours. 



5 c.c. trypsin, 20 c.c. 13 % alcohol, 



6 c.c. trypsin, 20 c.c. water, 

 Digestion 1-25 hours. 



5 c.c. trypsin, 20 c.c. 14 % alcohol, 



5 c.c. trypsin, 20 c.c. water, 



1-2 g. fibrin. Digestion 2-5 hours. 



5 c.c. trypsin, 20 c.c. 14 % alcohol, 



6 c.c. trypsin, 20 c.c. water, 

 Digestion 1 hour. 



5 c.c. trypsin, 20 c.c. 14 % alcohol, 

 5 c.c. trypsin, 20 c.c. water, 

 1 g. fibrin. Digestion 2 hours. 

 5 c.c. trypsin, 20 c.c. 14 % alcohol, 

 5 c.c. trypsin, 20 c.c. water. 

 Digestion 1 hour. 



These experiments are sufficient to show that alcohol, when present in 

 percentages varying from 3 to 7, has a very marked inhibitory effect 

 on the digestion of fibrin by trypsin but no such effect on the digestion 

 of caseinogen. The amount of fibrin digested under these conditions varied 

 from about 25 to 50 % of the amount digested in absence of alcohol, the 

 proportion varying somewhat with different trypsin solutions and with vary- 

 ing percentages of alcohol. In no case was there any appreciable difference in 

 the amount of caseinogen digested, beyond the limits of experimental error. 



With higher percentages of alcohol the digestion of fibrin was in some 

 cases entirely stopped, a fair amount of caseinogen still being digested, how- 

 ever. 



Digestion in c.c. 

 of N/10 nitrogen 



13. (o) 1 c.c. trypsin, 20 c.c. 25 % alcohol, 20 c.c. 1 % NajCOg 



(6) 1 c.c. trypsin, 20 c.c. water, 20 c.c. 1 % NagCOg 



1 g. fibrin. Digestion 3 hours, 



(o) 1 c.c. trypsin, 20 c.c. 25 % alcohol, 20 c.c. caseinogen 



(6) 1 c.c. trypsin, 20 c.c. water, 20 c.c. caseinogen 

 Digestion 1 hour. 



(222) 



1-3 



27-2 



200 

 23-7 



