EFFECT OF ALCOHOL ON TRYPSIN DIGESTION 27 



* Digestion in cc. 



of 2^/10 nitrogen 



14. (a) 1 CO. trypsin, 20 cc. 25 % alcohol, 20 c.c. 1 % NejCOs 4-8 



(6) 1 cc. trypsin, 20 cc water, 20 cc 1 % NajCOs 25-2 



0-8 g. fibrin. Digestion 3 hours. 



(«) 1 cc. trypsin, 20 cc 25 % alcohol, 20 cc caseinogen 32-8 



(6) 1 cc. trypsin, 20 cc water, 20 cc caseinogen ... ... 37-9 



Digestion 1*75 hours. 



16. (a) 1 cc trypsin, 20 cc 50 % alcohol, 20 cc 1 % NajjCOg 0-0 



(6) 1 cc trypsin, 20 cc water, 20 cc 1 % NajCOa 23-6 



1 g. fibrin. Digestion 3 hours. 



(a) 1 cc trypsin, 20 cc. 50 % alcohol, 20 cc caseinogen 5-3 



(6) 1 cc trypsin, 20 cc. water, 20 cc. caseinogen 25-2 



Digestion 1 hour. 



16. (a) Ic.c trypsin, 20 cc 50 % alcohol, 20 cc. 1% NajCOs 0-0 



(6) 1 cc trypsin, 20 cc water, 20 cc 1 % NaoCOg 23-4 



1 -2 g. fibrin. Digestion 3 hours. 



(a) 1 cc. trypsin, 20 cc 50 % alcohol, 20 cc caseinogen ... ... 4-6 



(6) 1 cc trypsin, 20 cc. water, 20 cc caseinogen ... ... 28-2 



Digestion 1 hour. 



Thes« experiments show that in presence of 25 % of alcohol the digestion 

 of fibrin by trypsin is entirely inhibited, while digestion of caseinogen still 

 proceeds to a limited extent. In presence of 12 % alcohol the amount of 

 fibrin digested is from 10 to 20 % of the control, while the caseinogen digested 

 amounts to about 85 % of the control. 



Trypsin is well known to be very unstable under some circumstances, and 

 it was considered possible that contact with dilute alcohol for some time 

 might lead to an actual destruction of that part of the enzyme molecule 

 which digests fibrin. The following experiments were carried out to test such 

 a theory. 



Digestion in cc 

 of N/IO nitrogen 



17. (a) 20 cc. trypsin, 15 cc. 15% alcohol! , , , „„„ „ , „ , 



/I^ «-. . ■ ,. / } kept at 37° C. for 3 hours 



(o) 20 cc. trypsin, 15 cc water ) 



2 cc of (a), 40 cc 0-5 % NagCOa 31-2 



2 cc of (6), 40 cc 0-5 o/o NagCOs 31-0 



1 '3 g. fibrin. Digestion 3 hours. 



18. la) 20 cc trypsin, 5 cc 30 % alcohol I , „„„ ^ r 



,' „ .. • ^ 1 [ kept at 37° C. for 3 hours 



(o) 20 cc trypsin, 5 cc water ) ^ 



2 cc of (a), 40 cc 0-5 % NaaCOg 18-2 



2 cc of (6), 40 cc 0-5 % NagCOg 18-3 



1-2 g. fibrin. Digestion 2 hours. 



lit. (a) 40 cc. trypsin, 10 cc 30 % alcohol I , ^ . .,.,0 n r ou 



/\x AC. 4- • in / \ kept at 37° C. for 3 hours 



(0) 40 cc trypsin, 10 cc water ) 



1 cc of (a), 40 cc 0-5 % NaaCOa ... 14-0 



1 cc of (6), 40 cc 0-5 % NaaCOg 14-2 



1 g. fibrin. Digestion 3 hours. 



;0. (a) 15 cc. trypsin, 10 cc. 15 % alcohol 1 , , , .,_o ^ , „ . 



,,,,„..,- / > kept at 37° C. for 3 hours 



(0) lo cc trypsin, 10 cc. water ) '■ 



2 cc of (o), 40 cc 0-5 % NagCOg 17-4 



2 cc of (6), 40 cc 0-5 % NagCOs 17-0 



1 g. fibrin. Digestion 2-75 hours. 



(223) 



