THE MEASUREMENT OF THE RESERVE ALKALINITY 



1. Titration Methods 



There are several methods by which the kal in . 



may l>c measured. The Bimplesl in theory <-"i 

 standard acid must be added t<> a measured quantil 

 order to reach the aeutral pohil as judged by change in tint 

 indicator. The indicators emplo} ed 

 change their tints ;ii Minn concentrations tl 

 of neutrality (i. e., at a high C H or Iom I' To bring th 



poinl of neutrality the added alkali will 1 d to neutral i 



bicarbonate of the plasma, bul other acid-bindii - 

 This will give us a false iinptvssiun of the acid-bin< 

 plasma, since, at the normal Ch of tin- blood, pr< I 

 tn anything like tin' extenl they do at higher deg I \ 



objection to the method is thai the proteins in1 

 9 of tin- indicators. 



The objections can be removed by determining the end p 

 metrically <»r by indicators thai change tint at aboul P 7 

 practical way is 1<> determine the change in Ch produced by 

 known volume of standard arid to blood plasma. I 

 in Ch will then be greater the less the alkaline I 



metric method irregularities thai mighl be 

 of carbonic arid in the blood to start with 

 the < '< » from the plasma after adding i 'I 



therefore consists in mixing 1 c c. plasma with 2 c 

 separating runnel, which is then 

 after which the fluid is transferred to a hyd 

 measured see pag< In normal blood this - P 



In acidosis, where there is a depleted alkali 

 will cause a much greater change in C« in diab< 

 or luw er. 



The technic involved in the abo> e meth< 

 routine clinical work. For such purp< - 

 and Rom nt ree maj be emploj ed. 



Tin M i thod op Levy R At 



lonsol" -lass of aboul 2 

 powdered ru utral potassium oxalal 

 immediately stoppered and placed on i 



the bl 1 are then placed ii 



and allowed to stand for ! ; ' 



