ACID 





incurred will become greatlj ag 

 duced in drawing the specimen for analysis Bui thi 

 this method has no1 been extensi ely emplo 

 Slyke, is the technical difficulty of making the nee 



It is most satisfactory to colled venous bl I 



a1 leasl of muscular resl so thai thei i CO oul 



venous stasis, and to centrifuge withoul permitting sideral 



of carbonic acid. The latter precaution is nec< 

 migration of acid radicles, e.g., HC1, from plasma ii I 

 tin' ('(>.. of iIip former is increased, and in the n on when the 



C0 3 is decreased, [f the CO a in the blood w< I I &me durii 



trifuging as it is in the body, the separate plasma would no1 

 same amount of alkali i. e., iis reserve alkalinity would I • 

 Although tl retically, therefore, centrifuging should !"■ p I in 



l"i({. 10. — Diagram of a 

 l" ads m tin- bottle cond< 

 filled with expired air, si 

 rotated so that tin- hluo.i a film o 



an atmosphere containing the same partial pr< CO 



the body (i. e., the alveolar air skx : _■ 144 this im- 



practicable for genera] use, and is unnec 



B] mini of blood is prevented bj allowing it to tlow 



very slowly 'Without anj suction . It is mixed in t 1 ith 



powdered (neutral potassium oxalat< enough to i 



solution with the blood . and immediately delivered i 1 ' 



tube under paraffin oil, which by floating on I 



fi ee diffusion of CO to the outside air e\ en the. 



more I than wain- . To mix the Mood with tl 



should be moved backward and forward Beveral 



shaken. 



A itrr cenl rifuging, aboul 3 

 w ith < '< >. al the Bame tension as in al> i 



