I III i:i.i»ol» il ll. 



purposeful division in order to increase the total kuH 



cells. The ultimate fate of the red ■••■ll fragmenl »wn. I - 



reasonable t<> suppose that the fragmented bil 



are carried to tin- liver, where the hemoglobin is ti 



hematin and l»il<- pigments. 



Hemolysis 



Another method of red blood cell destruction, which 

 not take place normally, is by hemolysis. The natu 

 tion of the hemoglobin with the stroma of the red eel . 

 marked, is no1 definitely known. Thai il is not mer< 

 Bac is shown by the fad thai the '•••11 may be <-ut into hits withi 

 hemoglobin being set free. In some manner the hemoglobin 

 ically bound with the Btroma of the red ••••II. I hich it c 



freed by a number of physicochemical and chemical 

 ess is known as hemolysis, and the substances which bring it 

 known as hemolytic agents. The manner in which tin 

 the release of hemoglobin from the blood is quite \ati>-<! 



1 1" the osmotic pressure of the plasma is lowered by « 1 i 1 1 1 1 i • 

 Bure within the corpuscle remains high, and water is absorbed 

 cell. If this absorption is sufficient, the cell ruptures and the hem< -• 

 is discharged. For this reason it is necessary in diluti 



solutions of salt having an osmotic pressure equal to tl 



hi 1 to proteel the red <-<'ll from hemolysis. This is 



a 0.9 per '•'•nt solution of sodium chloride Betti 

 however, by using either Ringer's solutioi 



per cenl CaCl„ and 0.03 per cenl KC1 or I ke's solution 



NaCl, 0.024 per cenl CaCl . 0.042 per cenl K( ' 0.01 

 \'al !< !( I and 1 per cenl gli s< 



In normal corpuscles hemolysis occurs I small it in - 



tions containing aboul 12 per cenl odium chl li 



diseases the fragility of the corpus 



The membrane and Btroma of the erythi 

 terial which is soluble in alcohol 

 Addition of these agents to the 1»1<»<><1 brinfi 

 ably by dissolving the li|><»i<lal material 

 occurs with saponin is similar in tj 

 lipoids, tin- compound being Boluble in v 



The hemolytic properl • rum, whether I 



normally presenl w hen the blo< 

 ln> produced artificially by the injection 

 subjed of great il 



