10 



A second culture, B. kiliensis, was atypical only in lack of the 

 characteristic pigment, which was revived on special media to the 

 violet red color without the usual green luster. These cultures 

 differed from B. prodigiosus as follows : 



Morphologically larger, rodlets of a young potato culture 

 2,5 5 // in length, 0,60,8 /< in diameter; rodlets from an old 

 potato culture may reach 8,0 /i in length. Motility appears in 

 4 hours after inoculation on potato at 35 C, in 24 hours at room 

 temperature. Gelatin is rapidly liquefied, with a thick orange red 

 surface pellicle. B r e u n i g described the growth on potato as at 

 first sealingwax red and later like that of B. prodigiosus. My 

 cultures showed in 24 hours a slight growth of a red violet color, 

 which became luxuriant and darker until, in 10 days, it was heavy, 

 corrugated, and looked like iron rust. Green luster is often 

 seen. Bouillon cloudy, thick orange red pellicle, and red sedi- 

 ment. The production of gas by this form, so far as I can find, 

 has not been previously determined. It occurs readily in dextrose, 

 lactose, and sucrose bouillon, and unlike that of the forms so far 

 described, is only in small part (2028 ; ) C0 2 . In standard 

 asparagin solution with 1,0 / dextrose, gas to 14,3 of the tube 

 length was formed, none of it C0 2 . Development with pigment 

 occurs at 37 " C. The pigment is violet red and lacks the orange 

 red surface growth seen at room temperature. Nitrates are reduced 

 to nitrogen gas. Odor not characteristic. 



B. miniaceus (Zimmermann). 



Isolated in 1889 from water, by Zimmermann (59), who 

 has suggested that the organism seems identical with Dowdes- 

 well's B. rosaceus metalloides. Zimmermann described 

 it, however, and gave it the above name. Migula, commenting 

 on this form, said: "Zimmermanns Vermutung, dafi diese Art 

 identisch mit B. rosaceus metalloides ist, diirfte nicht rich tig 

 sein. Er schliefit sich eher an den Kieler Bacillus an." 



Hoagland Laboratory, Brooklyn, furnished a culture, isolated 

 from water, which seemed identical with the B. miniaceus from 

 Krdl, except for a tendency to lose power of pigment production. 

 A second culture from Hoagland Laboratory, evidently identical 

 with the first, came into my hands as "B. rubrus", was colorless, 

 and could not be made to regain the chromogenic power. This 

 culture produced the typical amount of gas, 4045 / . 



My cultures of B. miniaceus are more like B. ply mou- 

 th en sis than like B. kiliensis. Contrary to Zimmermann, 

 I find the bacillus motile in young cultures. Liquefaction of gelatin, 

 slower than B. plymouthensis, but not so slow as stated by 

 Zimmermann, i. e. begins within 5 days, and is complete in 

 three or four weeks. The liquid is red. Potato and agar, like 

 B. plymouthensis, metallic luster rarely seen and only on 

 glucose agar. Gas production like B. plymouthensis. Nitrates 

 reduced to nitrites only. No fecal odor. 



