

SPECIAL STAINS ' IOI 



This complex stain represents -a type *of v which Jenner's, Leish- 

 man's, and Romanowsky's are members. To use this stain, a 

 blood or pus film is spread and air-dried. The stain is then run 

 on the slip, or slide, for one minute. After this time slowly drop 

 distilled water in quantity similar to that of stain used. This is 

 when the true staining takes place. After three minutes wash in 

 distilled water, dry and mount. Nuclei, malarial parasites, try- 

 panosomes, and bacteria are stained blue; red cells are stained 

 pinkish-orange, while the granules of the leucocytes are stained 

 pink, lilac, or blue, depending upon their character. 



Giemsa's Stain 



This stain is used for demonstrating the organism of syphilis, 

 trypanosomata, granules and the like, and is prepared as follows : 



Azur II Eosin 3 grams. 



Azur II 8 grams. 



Glycerine C. P 250 c.c. 



Methyl alcohol 250 c.c. 



Bacteria are often covered with capsules that are difficult to 

 stain, and special methods have been devised to demonstrate 

 them. 



1. Air-dry the specimen. 



2. Harden and fix in absolute methyl alcohol. 



3. Dilute stain with distilled water, using one drop of stain to each cubic 

 centimeter of water. 



4. Cover preparation with dilute stain fifteen minutes to three hours 

 (longer period for spirochaetesj. 



5. Wash in running water. 



6. Blot and mount. 



Capsule Staining 

 Welch's Method. 



1. Cover-glass preparations are made in the usual manner, and over the 

 film after fixing, glacial acetic acid is poured. 



2. Without washing off the acid, aniline water gentian violet is poured on. 



