

STAINING DIPHTHERIA BACILLI 10$ 



Neisser's method of staining the diphtheria bacillus. 

 Two stains are needed (Fig. 21): 



FIG. 2i. B. Diphtheria stained by Neisser's method. (Williams.) 



The staining steps are as follows: 



1. Prepare film, fix and dry. 



2. Pour on "A" for thirty seconds. 



3. Wash well in water. 



4. Dry and pour on "B" for thirty seconds. 



5. Wash, dry and mount. 



The protoplasm of the bacilli will be stained brown, and the 

 characteristic (diagnostic) chromatin points will be stained a 

 deep blue black. 



