xii CONTENTS 



CHOLERA 



Inoculation of Gelatine, Agar-Agar, and Broth with 



Various Vibrios 37, 38 



STAINING OF FLAGELLA (VAN ERMENGEM) 



(a) Bacillus of Typhoid Fever . . . . . . 38, 39 



(b) Vibrio cholera Asiatics? 38, 39 



LESSON IX 



CHOLERA (continued) 



Examination of Vibriones choleras Asiaticse, Finkler- Prior, 



and Metchnicovi in Cultures 40 



STAINING OF VIBRIOS 



(a) Gentian-Violet 40 



(Z>) Fuchsine 40 



SPIRILLUM RUBRUM 



(a) Staining 41 



(b) Gelatine Stab Cultures 41 



Hanging Drop Cultures of Vibrio choleras Asiaticse . . 41 



CHOLERA VIBRIOS IN THE ANIMAL BODY 



(a) Peritoneal Fluid of Guinea-Pig 41 



(b) Separation of Vibrios by Plate Cultures . . .42, 43 

 ACTION OF SUNLIGHT ON THE VIBRIO CHOLERA ASIATICS . 43 



LESSON X 



CHOLERA (concluded) 



Examination of Plates 44 



Impression Specimens 44 



Varieties of Choleraic Vibrios 44, 45 



