CONTENTS xi 



PAGE 



ACID FORMATION BY VIRULENT AND ATTENUATED BACILLI. 23 



METHODS OF ATTENUATION ....... 23 



STAINING OF THE BACILLUS ANTHRACIS 



(a) Loffler's Methylene-Blue 23 



(6) Gentian-Violet 23 



(c) Gram's Method 24 



LESSON VI 



BACILLUS ANTHRACIS (continued) 



Hanging Drop Cultures (Staining) 25 



Asporogenous Cultures (Staining) 25 



Impression Specimens .26 



Examination of Fresh Tissues by Means of Films 

 Spleen of Mouse 27, 28 



LESSON VII 



BACILLUS ANTHRACIS (continued) 



Plate Culture Method . . . . ' . . 29, 30 

 Staining of Spores . 30-32 



Ehrlich's, Loffler's, or Ziehl's Fuchsine .... 30-32 



SEPARATION OF HAY BACILLUS OUT OF HAY INFUSION 33 



LESSON VIII 



BACILLUS ANTHRACIS (concluded) 



Impression Specimens (Gelatine Plates) .... 34 



STAINING OF TISSUES IN FROZEN SECTIONS 



(a) Loffler's Methylene-Blue 35 



(&) Methylene-Blue and Eosine 35, 36 



(c) Methylene-Blue and Picrocarmine ... 36 



(d) Gram's Method . - - , . . . 36, 37 



