28 GENERAL BACTERIOLOGY LESSON vi 



(3) Stain a film with eosine and methylene-blue. 



(a) Dip the cover-glass for a few seconds in a wide- 

 necked pot containing *5 per cent solution of 

 eosine in 50 per cent alcohol. 



(b) Wash it in water, and dry it with blotting-paper. 



(c) Pass it three or four times through the flame, and 

 now stain quickly in Loffler's methylene-blue. 



This method stains the eosinophile granules of the 

 leucocytes and also brings out the red corpuscles. 



(4) Stain a film according to Gram's method, first passing 

 the specimen through acetic acid. Counterstain with eosine. 



In each case mount in xylol balsam and examine with 

 % in. oil immersion. 



Note the absence of spores and the relation of the 

 bacilli to the leucocytes. 



