LESSON VII 



Bacillus Anthracis (continued} Plate Cultures Staining of Spores- 

 Separation of Bacillus Subtilis from Hay Infusion. 



Bacillus Anthracis (continued) 



EXAMINE the litmus agar-agar cultures of Bacillus anthracis 

 made on the last occasion. 



Note the difference in tint of the two tubes. 



Plate Culture Method 



(a) Take a broth culture of Bacillus anthracis (forty- 

 eight hours old) and three gelatine tubes. 



(6) Liquefy the gelatine tubes in the warm incubator. 



(c) Shake the broth culture, and from it inoculate the 

 first gelatine tube with three platinum loops, and label it I. 



(d) From I., after shaking, inoculate the second gelatine 

 tube with three platinum loops, and label it II. 



(e) From II. similarly inoculate the third tube, and label 

 it III. 



(/) Take three sterilised Petri's capsules and place them 

 on moist filter-papers, and label them I. II. and III. 



(g) Now heat the mouths of I. II. and III. in the flame 

 and allow them to cool. 



