30 GENERAL BACTERIOLOGY LESSON 



(h) Pour out each gelatine tube, after gently shaking it, 

 into the corresponding capsule. 



(i) Allow the gelatine to set in a uniform layer, and 

 when set, place the capsules in the cool incubator. 



Examine on the next occasion : colonies will appear 

 on each plate, naturally most numerously on plate 

 I. and least on plate III. (vide infra p. 34). 



Staining of Spores 



Bacillus filamentosus, or anthracis, or megatherium (agar- 

 agar or potato cultures). 



Solutions required : 



(a) Ehrlich's, Loffler's, or Ziehl's fuchsine. 



(/3) Acid alcohol : alcohol 97 cc., hydrochloric acid 3 cc. 



(7) Loffler's methylene-blue. 



EhrlicKs fuchsine : Aniline water 100 cc. 



Concentrated alcoholic solution of 

 fuchsine 11 cc. 



This staining solution does not keep well, and there- 

 fore only small quantities should be made at a 

 time. 



Loffler's fuchsine : Aniline water 100 cc. 



Fuchsine crystals 4 to 5 grammes. 



1 per cent solution of caustic soda 1 cc. 



Shake till dissolved (about half an hour). 



