' 3? GENERAL BACTERIOLOGY LESSON 



(h) Now counferstam with Loffler's methylene-blue, wash 

 in water, dr^ and mount. 



Examine with ^ ^ n - ^ immersion : the bacilli are 

 Jilue and the spores red. 



Make a drawing. 



The spores of the Bacillus filamentosus and the 

 Bacillus megatherium are more easily stained than 

 those of the Bacillus anthracis. 



Second Memod. 



(a) Prepare a film in the usual manner. 



(b) Pass it three times through the flame. * 



(c) Place it for two mrftutes in absolute alcohol, and for 

 further two minutes in chloroform. 



(d) Wash the film in water, dry it between blotting- 

 paper, and pass it again three times through the flame. 



(e) Leave it for two minutes in a 5 per cent solution of 

 chromic acid, and then wash it in water, and dry it between 

 blotting-paper. 



(/) Once more pass ^^tree times through the flame, and 

 place it in a warm solution of carbol fuchsine for five to 

 fifteen minutes. 



(g) Rinse the film in water, and decolourise in 5 per cent 

 sulphuric acid, till the specimen is faintly pink. 



(h) Wash it in water and counterstain in malachite 

 green. 



(i) Wash again, dry and ftiount. 



t 

 The spores are red and well stained, the bacilli green. 



