VII 



SEPARATION OF THE HAY BACILLUS 



Separation of the Hay Bacillus (Bacillus siibtilis) 

 out of Hay Masion. 



Six tubes containing hay infusion are suppliec^ 

 Heat them for five* ;ten, fifteen, twenty, twenty-five and 

 thirty minutes respectively in water at -80 C. 



From each tube inoculate a broth Hibe with three 

 platinum loops of the* infusion and label them. 



Keep the six tribes in the cool incubator and examine 

 them on the next occasion. 



Pure cultures of the hay bacillus will probably be 

 obtained. 



