LESSON VIII 



Bacillus Anthracis (concluded) Impression Specimens (Gelatine Plates) 

 Staining of Anthrax Bacilli in Tissues Staining of Flagella. 



Bacillus Anthracis (concluded] 



WITH a dissecting microscope examine the plates, made on 

 the previous occasion, for colonies of anthrax bacilli. 



Observe the characteristic appearance of the colonies. 



Now place a clean cover-glass over a colony and examine 

 it with a high power. 



Make a drawing. 



Impression specimens. 



(a) Take a perfectly clean cover-glass, sterilise it by pass- 

 ing it through the flame, and place it over a suitable colony. 



(b) With a needle carefully press the cover-glass down, 

 until the colony is slightly flattened out. 



(c) With a sterilised needle lift the cover-glass with the 

 colony adhering to it from the surface of the gelatine ; then 

 proceed as described before (vide p. 26). 



