LES. vin STAINING OF ANTHRAX BACILLI IN TISSUES 35 , 



Staining of Tissues for Anthrax Bacilli 

 (Frozen Sections) 



(1) Simple staining with Lo'ffler's methylene-blue. 



Tissues supplied : lung, kidney, liver and spleen of a 

 mouse dead of anthrax. 



(a) Place a section in Loffler's methylene-blue for five 

 minutes. 



(b) Wash it in distilled water for half a minute. 



(c) Then decolourise it for a quarter to half a minute 

 with very dilute acetic acid (one to two drops of glacial 

 acetic acid to a watch -glassful of water), till the section 

 is pale blue. 



(d) Again wash it in water for half a minute. 



(e) Now place it in absolute alcohol for half a minute to 

 one minute. 



(/) Clear the section in xylol. 1 



(g) Transfer the specimen to a slide with a piece of 

 cigarette-paper. 



(h) Mount it in xylol balsam. 



Examine it with low and high powers and y 1 ^ in. 

 oil immersion. 



(2) Double staining with methylene-blue and cosine. 

 Prepare the following solution (Czinzinski's solution) : 



1 Instead of xylol other clearing media may be used, as bergamot 

 oil, a mixture of turpentine and creasote, or cedar-wood oil. 



