38 GENERAL BACTERIOLOGY LESSON 



Place the gelatine tubes in the cool incubator at 22 C., 

 the others in the warm incubator at 38 -5 C. 



Staining of Flagella (Van Ermengem) 



Prepare the following solutions : 



(a) Osmic Acid (2 per cent solution) 1 part. 

 Tannin (10 to 25 per cent solution) 2 parts. 



To each 100 cc. of the tannin solution add four or 

 five drops of glacial acetic acid. 



(/3) Nitrate of silver (-25 to -5 per cent solution). 



(y) Gallic Acid ... 5 grammes. 

 Tannin ... 3 grammes. 

 Fused acetate of soda . 10 grammes. 

 Distilled water . .350 cc. 



Boil the cover-slips to be used in the following solution : 



Potassium bichromate . 60 grammes. 



Concentrated Sulphuric Acid 60 grammes. 

 Water .... 1000 cc. 



Then wash them repeatedly in water. Keep them in 

 absolute alcohol and before use allow them to dry, without 

 wiping, by placing them in a vertical position, protected 

 from dust. * 



Bacillus of typhoid fever and Vibrio cholem Asiatic. 



Carefully suspend one or two loops of an agar-agar culture 

 (ten to eighteen hours old) in a watch-glassful of distilled 

 water. 



