LESSON IX 



Examination of Vibriones Choleras Asiaticse, Finkler - Prior, and 

 Metchnicovi in Cultures and in the Animal Body Plate Cultures 

 Germicidal Action of Sunlight Hanging Drops. 



Cholera (continued) 



(1) EXAMINE and compare the tubes inoculated with 

 Vibrio cholerse Asiaticse, Vibrio Finkler-Prior, and Vibrio 

 Metchnicovi respectively. 



(2) Prepare cover -glass specimens of Vibrio choleras 

 Asiaticse, Vibrio Finkler-Prior, and Vibrio Metchnicovi in 

 the usual manner. 



Stain them with dilute aqueous gentian- violet or dilute 

 aqueous fuchsine, as follows : 



(a) Filter some of the staining solution into a watch- 



(5) Stain for about five to ten minutes, previously 

 clearing the specimen with acetic acid, if necessary. 



(c) Wash in water, dry and mount. 



Examine with ^ in. oil immersion and compare the 

 three forms. 



