LESSON X 



Vibrio Cholerse Asiatics (concluded) Impression Specimens from Plates 

 Comparison of Different Varieties Curdling Ferment 

 Actinomycosis Cladotlirix Staining of Tissues embedded in 

 Celloidin Flagella Staining after Pitfield. 



Cholera (concluded) 



(1) EXAMINE the plates made from the heart's blood of 

 the guinea-pig. 



The colonies may be so small that they can only be seen 

 with a magnifying-glass. 



Select a typical colony and make a gelatine stab culture 

 from it which should be kept at 22 C. 



Prepare also impression specimens of suitable colonies, 

 and stain them with aqueous gentian-violet. 



Examine with a high power and T ^ in. oil im- 

 mersion. 



(2) Inoculate five gelatine tubes from the five varieties 

 of cholera vibrios supplied (stab cultures). 



Place them in the cool incubator at 22 C. 



Compare their mode of growth and rate of lique- 

 faction from day to day. 



