xi PUS AND GONORRHCEA 49 



(/) Dry them between blotting-paper and again pass 

 them three times through the flame. 



(g) Now stain them in the usual manner with dilute 

 aqueous gentian-violet, or by Gram's method. 



Mount in xylol balsam and examine with -% in. oil 

 immersion. 



(9) Prepare specimens of the iresh pus supplied which 

 contains Staphylococci. 



Prepare thin films in the usual manner, clear them in 

 acetic acid, and stain with cosine and methylene-blue in 

 the following way (vide p. 28) : 



(a) Stain with cosine. 



(b) Wash, and dry with filter-paper. 



(c) Heat again and stain with Loffler's methylene-blue. 



(d) Wash, dry and mount. 

 Examine with ^ i n - ^ immersion. 



(10) In the same manner prepare and stain specimens of 

 gonorrhoeal pus. (Eosine and methylene-blue or gentian- 

 violet.) 



Examine with T ^ in. oil immersion : note the cocci 

 in the cells. 



Stain another specimen by Gram's method, counter- 

 staining with eosine, and contrast with previous specimens. 



The gonococci do not retain the stain. 



