LESSON XII 



Pyogenic Cocci (concluded) Staining of Hanging Drops Staining in 

 Tissues Fibrin Staining in Croupous Pneumonia. 



Pyogenic Cocci (concluded) 



(1) STAIN the hanging drop cultures of the Streptococcus 

 pyogenes prepared on the previous occasion 



(a) with dilute fuchsine ; 



(b) by Gram's method. 



Mount in xylol balsam and examine with a high 

 power. 



(2) Examine unstained the broth cultures, of the three 

 Streptococci, made last time. 



(a) Shake the tube, and with a sterilised loop remove a 

 drop of the fluid, and place it on a clean cover-glass. 



(b) Carefully place the cover-glass on a clean slide and 

 with a brush paint some vaseline round the margin of the 

 cover-glass, so as to prevent evaporation. 



Examine with a high power and ^ in. oil immersion, 

 using a narrow diaphragm. 



