xiii DIPHTHERIA AND LEPROSY 57 



the serum tube at 38-5 C. and the gelatine tube at 

 22 C. 



(2) Prepare cover-glass specimens from an agar-agar 

 culture and stain with aniline gentian-violet. 



(a) Leave them in the stain for five to ten minutes. 



(b) Decolorise in spirit. 



(c) Wash in water, dry and mount. 



Examine with ^ i n - ^ immersion : note the clubbed 

 forms and the characteristic grouping. 



(3) Diphtheritic membrane. 



Stain sections of diphtheritic membrane with the 

 eosine methylene-blue mixture in the usual manner (vide 

 p. 35). 



Examine with low and high powers. 



(4) Prepare hanging drop cultures in broth and keep 

 them at 38 -5 C. 



Examine them from day to day, so as to study the 

 peculiar forms which the diphtheria bacillus often 

 shows. 



Leprosy Tissue 



(1) Stain with picrocarmine, and then according to 

 Weigert's fibrin method (vide p. 51). 



Examine with low and high powers and ^ i n - il 

 immersion, and note the arrangement of the 

 bacilli and their relation to the cells. 



(2) Stain a section with a filtered carbol fuchsine solution. 



