58 GENERAL BACTERIOLOGY LESSON xin 



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(a) Leave in the stain for twenty to thirty minutes. 

 , (b) Wash it quickly in -6 per cent saline solution. 



(c) Decolourise it in hydrochloric acid (25 per cent) for 

 a few seconds, until the red tint disappears. 



(d) Transfer the section to 70 per cent alcohol, and with 

 a needle move it about till no more red is washed out. 



(e) Remove the alcohol with '6 per cent saline solution. 



(/) Now counterstain the section in Loffler's methylene- 

 blue for one to two minutes. 



(g) Wash thoroughly in -6 saline solution. 



(h) Dehydrate in absolute alcohol. 



(i) Clear in xylol and mount in xylol balsam. 



Examine with low and high powers and -^ i n - oil 

 immersion. The bacilli are stained red and the 

 tissues blue. 



