72 GENERAL BACTERIOLOGY LESSON 



the centre of a clean cover-glass, sterilised by passing it 

 several times through the flame. 



(b) Place the cover-glass, with the drop downwards, on a 

 moistened ring of blotting-paper (vide Fig. 1). 



Examine the drop with a high power and with y^ in. 

 oil immersion for phagocytosis (vide footnote p. 10). 



(2) Examination of stained specimens. 



(a) Spread some of the lymph or peritoneal fluid uni- 



FlG. 1. 



a, Cover-glass ; 5, moistened ring of blotting-paper ; c, slide ; d, drop 

 of lymph or peritoneal fluid. 



formly over several cover-glasses, and allow the films to dry 

 in the air. 



(b) Clear in acetic acid in the usual manner. 



(c) Wash in water, dry, and pass three times through the 

 flame. 



(d) Stain with Loffler's methylene-blue, wash, dry and 

 mount. 



Examine with ^ i n> ^ immersion for phagocytosis. 

 (3) Examination of double-stained specimens. 



(a) Prepare films as above. 



(b) Stain with a solution of cosine (-5 gramme in 100 cc. 

 of 50 per cent alcohol) for half a minute, without previously 

 clearing in acetic acid. 



