74 GENERAL BACTERIOLOGY 



Examine with low and high powers. It will be seen 

 that the anthrax bacilli had entered the circulation. 



(5) Effect of heat on phagocytosis in the frog. 



(a) Inoculate a pithed 1 frog with a broth culture of 

 Bacillus anthracis as before, and keep it in. a moist chamber 

 at 30 to 35 C., covering it over with moistened blotting- 

 paper. 



(b) Examine the lymph six to eighteen hours later, by 

 means of cover-glass films, stained with methylene-blue in 

 the usual manner. 



It will be seen that the bacilli have grown luxuriantly, 

 and that there is no phagocytosis. 



Other films should be prepared and stained with 

 cosine and methylene-blue. 



It will be seen that there are very few eosinophile 

 cells. 



1 In this experiment the cord alone should be destroyed. 



