LESSON XVI 



Phagocytosis (concluded) Effect of Anaesthesia Phagocytosis in the 

 Hanging Drop Chemiotaxis Diagnosis of Diphtheria. 



Phagocytosis (concluded} 

 (1) Heated frog. 



(a) Remove, with a capillary pipette, some lymph from 

 the inoculated thigh of a frog kept at 30 to 35 C. for 

 twelve to eighteen hours (vide p. 74). 



Examine it as a hanging drop with a high power, 

 (vide p. 72). 



Note that the anthrax bacilli have grown luxuriantly, 

 and that there is no phagocytosis or " eosinophile 

 leucocytosis." 



(b) Prepare films of this lymph, dry and stain them 



(1) with Loffler's methylene-blue ; 



(2) with eosine and methylene-blue. 



Examine them with a high power and ^ in. oil 

 immersion. 



(c) Now take the frog out of the incubator and keep it 

 cool for twenty-four to forty-eight hours. 



