LESSONS I AND II 



Cleaning and Sterilisation of Tubes and Flasks Preparation of 

 Nutrient Media Beef Broth Glycerine Broth Grape-Sugar 

 Broth Meat Infusion Gelatine Grape-Sugar Gelatine 25 per 

 cent Gelatine Carbolic Acid Gelatine Peptone Solution Potato 

 Tubes Milk Tubes Agar-Agar Glycerine Agar-Agar Grape- 

 Sugar Agar-Agar Serum Tubes. 



Cleaning and Sterilisation of Test-Tubes, 

 Flasks, and Beakers 



I. CLEANING OF NEW TEST-TUBES. 



(1) Eoll a little cotton -wool round a strong glass rod 

 and fasten it with a piece of strong thread or string. 



(2) Dip the cotton -wool in strong nitric acid, and 

 thoroughly rub the inside of each tube. 



(3) Wash the tubes three or four times in water, to 

 remove all trace of the acid. 



(4) Allow them to drain, until they are nearly dry. 



(5) Rinse each tube with a little absolute alcohol or 

 strong spirit. 



(6) Again allow the tubes to drain. 



(7) When quite dry, they are ready for sterilisation. 



