LESSON IX 



Examination of Decomposing Meat Aerobic Putrefaction Anaerobic 

 Putrefaction How to Examine a Sample of Unsound Meat How 

 to Examine Meat for Trichina Spiralis Cysticercus Psorosper- 

 mosis Examination of Ice Cream. 



Examination of Decomposing or Diseased Meat 



A. PUTREFACTION. 



Mince a little fresh meat, and add small quantities of 

 it to five tubes containing sterile nutrient broth. 



(a) Aerobic putrefaction. 



(1) Place two tubes in the warm incubator. 



(2) After three to four days examine the contents of the 

 tubes microscopically by means of cover-glass films, and 

 prepare plates from each in the usual manner. 



(3) Work up the colonies as they appear. 



Various kinds of Proteus, amongst others the Proteus 

 Zenkeri, Bacillus coli communis, Torulse, Staphylo- 

 cocci, Sarcinse, etc., will be found. 



(b) Anaerobic putrefaction. 



(1) Place two of the tubes in a flask, which should be 

 exhausted of air in the manner already described. 



