LESSON X 



Examination of Antiseptics and Disinfectants Method of Testing 

 Antiseptics Methods of Testing Disinfectants Koch's Method 

 Carbolic Acid Mercuric Chloride Steinberg's Method of Testing 

 Antiseptics Disinfectant Action of Gases Sulphur Dioxide 

 Chlorine Ammonia. 



Examination of Antiseptics and Disinfectants 



A. METHOD OF TESTING ANTISEPTICS. 



Carbolic acid. 



(1) Prepare a series of carbolic acid broth tubes (1 : 100, 

 1 : 200, 1 : 300, 1 : 400, 1 : 500). 



To tubes containing 10 cc. of broth add respectively, 

 with a sterile graduated pipette, *1 cc., '05 cc., 

 03 cc., "025 cc., '02 cc. of liquid carbolic acid. 



(2) Inoculate these five tubes with a platinum-loopful of 

 a pure culture in broth of the bacillus of typhoid fever or 

 blue pus, twenty-four hours old. 



Inoculate also an ordinary broth tube with the same 

 organisms, for the purpose of control. 



(3) Place the six tubes in the warm incubator. 

 Examine and compare the tubes from day to day. 



