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BACTERIOLOGICAL ANALYSIS 



LESSON 



(3) Allow the gas to pass over one of the strips of cloth 

 for one to two hours. 



Fia. 14. Flask I contains some copper and sulphuric acid, which are heated over 

 the flame to prepare the sulphur dioxide ; II, Wash bottle containing water ; 

 III, Wide glass tube in which the infected strip of cloth is placed ; IV, Spiral 

 glass tube surrounded by a freezing mixture of salt and pounded ice ; V, 

 Small flask, placed in a beaker containing ice and salt. 



(4) Then remove it and drop it into a broth tube, 

 which is to be kept at 38 '5 C. for several days. 



FIG. 15. I, Flask containing sodium chloride (one part by weight), manganese 

 dioxide (one part by weight), and sulphuric acid and water (two parts by 

 weight of each), which must be very gently heated ; II, Wash bottle containing 

 water ; III, Wide glass tube containing the infected strip of cloth ; IV, 

 Collecting cylinder filled with water. 



Examine the contents of the tube for growth from day 

 to day, both microscopically and by means of plates. 



