LESSON XI 



Examination of an Animal dead of a Bacterial Disease Anthrax 

 Pyocyaneus Septicsemia Cholera Asiatica Rapid Method of 

 Embedding Tissues in Paraffin Examination of Typhoid Spleen. 



How to Examine an Animal dead of Bacterial Disease 

 Three animals are supplied : 



(a) mouse inoculated with Bacillus anthracis ; 



(&) guinea-pig inoculated intraperitoneally with 

 Bacillus pyocyaneus ; 



(c) guinea - pig inoculated intraperitoneally with 

 Vibrio choleras Asiatics. 



(1) Nail the animal out on a wooden board, thoroughly 

 washed with mercuric chloride 1 : 1000. 



(2) Moisten the hairy surface of the animal's abdomen 

 with spirit, and then wash it with mercuric chloride 1 : 1000. 



(3) With a pair of sterilised forceps and a pair of sterilised 

 scissors carefully reflect the skin from the abdomen and 

 chest. 



(4) Thoroughly cauterise the exposed surface with a red- 

 hot glass rod, and then open the chest and abdominal cavity 

 with sterile instruments 



