LESSON V 



Peptones and Albumoses (concluded} Separation of Albumoses. 

 Albumoses (concluded) 



SEPARATION or DEUTERO-ALBUMOSE FROM PRIMARY ALBU- 

 MOSES (proto-albumose and hetero-albumose). 



Prepare a neutral solution of Witte's "peptonum siccum" 1 

 in the least possible quantity of distilled water. 



(1) Add concentrated salt solution: precipitate (I.) 

 (primary albumoses). 



(2) Filter. 



(3) To filtrate add acetic acid, saturated with salt, as 

 long as a precipitate falls down : precipitate (II.) (mixture 

 of proto- with a little deutero-albumose). 



(4) Filter. 



(5) Collect precipitates (I.) and (II.) and set them apart 

 (vide infra (14)). 



(6) The filtrate, which contains the deutero-albumose, must 

 be placed in a dialysing membrane and be dialysed against 

 distilled water, to remove the acetic acid and the salt. 



1 Obtained from E. Merck of Darmstadt. 



