LESSON v SEPARATION OF ALBUMOSES 153 



A few crystals of thymol should be added, both to the 

 liquid in the membrane and to the water around. 



(7) Concentrate the contents of the membrane in vacuo 

 at 37 0. to 40 C., having previously added a few thymol 

 crystals. 



($) The concentrated solution should be poured slowly 

 into five to six times its volume of absolute alcohol : pre- 

 cipitate (III.) (deutero-albumose). 



(9) Allow the precipitate to stand under the alcohol for 



several days. 



(10) Syphon off the alcohol, and drive off the remainder 

 of the alcohol by keeping the precipitate at 40 C. 



(11) Dissolve the dry residue in the smallest possible 

 quantity of distilled water. 



(12) Pour this solution slowly into five to six times its 

 volume of absolute alcohol, and again allow the precipitate 

 which appears to stand under the alcohol for several days. 



(13) Separate the alcohol as before, and dry the pre- 

 cipitate in vacuo at 40 C. 



The dry residue is deutero-albumose. 



(14) To obtain the hetero-albumose from precipitate (I.), dis- 

 solve the latter in the least possible quantity of distilled water. 



(15) Dialyse against running water for twelve hours, and 

 then against distilled water for further two to foui hours, till all 

 the salt has been removed. (Add thymol crystals as above.) 



(16) Pour the dialysed liqufd from the membrane into a 

 beaker : if it contains a precipitate precipitate (IV.) this is 

 hetero-albumose. 



(17) Filter (vide infra (21)). 



