154 BACTERIOLOGICAL CHEMISTRY LESSON v 



(18) Wash the residue on the filter-paper with distilled water, 

 and then repeatedly with absolute alcohol. 



(19) Place it under alcohol for several days, in order to 

 dehydrate it completely. 



(20) Separate off the alcohol and dry as above. 

 The dry residue is hetero-albumose. 



(21) The filtrate from (17) should be evaporated to a small 

 bulk in vacuo at 40 C. 



(22) To the concentrated liquid add saturated salt solution : 

 precipitate (V.) 



(23) Filter. 



(24) Dry the residue at 40 C., and redissolve it in the least 

 possible quantity of distilled water. 



(25) Dialyse the solution, adding thymol crystals, as de- 

 scribed above. 



(26) Concentrate the contents of the membrane in vacuo at 

 40 C. 



(27) Pour the concentrated solution into an excess of 

 absolute alcohol, and allow the precipitate to stand under the 

 alcohol for several days. 



(28) Separate off the alcohol, and dry as above. 

 The dry residue is proto-albumose. 



Apply the tests and reactions described in Lesson IV. 

 to concentrated solutions of the albumoses. 



