LESSON VI 



Diphtheria Albumoses Diphtheria Bacilli Diphtheria Spleen. 



Diphtheria Albumoses (Sidney Martin) 



(a) Diphtheria bacilli. 



(1) To 200 cc. of sterile ox serum add 2-5 cc. of a sterile 

 solution of 10 per cent caustic soda and 60 cc. of non- 

 peptonised sterile broth. 



This must be done under strictly aseptic conditions. 



(2) Fill twenty-six tubes with this solution, using a 

 sterile 10 cc. pipette, and keep the tubes at 56 C. for five 

 to six days, for several hours each day. 



(3) Select the tubes which remain sterile, and inoculate 

 them with virulent diphtheria bacilli. 



(4) Keep them at 38-5 C. for twenty-four to thirty-two 

 days. 



(5) Pour the contents of tubes into a large excess of 

 strong methylated spirit (1000 cc.) 



(6) Allow the precipitate to settle, and to stand under 

 the spirit for a week or so. 



(7) Filter through paper. 



