156 BACTERIOLOGICAL CHEMISTRY LESSON 



(8) Extract the residue with cold distilled water by 

 means of shaking, until nothing more dissolves out. 



(9) Evaporate the watery extract to a small bulk at 

 40 0. 



(10) Throw this concentrated extract into an excess of 

 absolute alcohol : precipitate of albumoses. 



(11) Allow this to stand under absolute alcohol for 

 several days. 



(12) Pour off the alcohol. 



(a) Residue = albumoses (b) Alcoholic liquid : 



Evaporate to dryness at 40 C. 

 Extract residue several times 



with, absolute alcohol : 

 Deutero-albumose. 



(13) Mix (a) and (b) together, and dissolve in the 

 smallest possible quantity of distilled water. 



(14) Pour the watery solution into five to six times its 

 volume of absolute alcohol, and allow it to stand for several 

 days. 



(15) Pour off the alcohol and dry at 40 C. 



(16) Again dissolve in distilled water. 



(17) Pour this solution once more into alcohol, and 

 allow it to stand for several days. 



(18) Pour off the alcohol, dry, and redissolve in water, 

 and again pour into absolute alcohol. 



(19) Allow the precipitate to stand under absolute 

 alcohol for five to six weeks. 



(20) Pour off the alcohol, and dry the residue in vacuo 

 over sulphuric acid. 



