LESSON VII 



Diphtheria Toxine Action of Magnesium Sulphate and Ammonium 

 Sulphate on Sulphate of Quinine. 



Diphtheria Toxine (Uschinsky) 



(1) PREPARE a solution of aspartate of sodium : 



Water . . . 1000 cc. 



Glycerine . . . 35 cc. 



Sodium chloride . . 6 grammes. 



Calcium chloride . . '1 gramme. 



Magnesium sulphate . . '2-*4 grammes. 



Bi-potassium phosphate . 2-2-5 grammes. 



Lactate of ammonium . . 6-7 cc. 



Sodium aspartate . . 3 '4 grammes. 



(2) Mix thoroughly, and heat for half an hour at about 

 40 0. 



(3) Neutralise with sodium carbonate, and again heat 

 for half an hour. 



(4) Filter. 



(5) Pour the filtrate into flasks, and sterilise in the 

 steamer in the ordinary manner. 



