STREPTOCOCCUS EEYSIPELATIS 113 



Leber isolated the active principle from the cultures in 

 the form of a crystalline body, to which he gave the name 

 ofphhgosin, and which, if injected in small quantity, leads 

 to suppuration without the presence of micro-organisms. 

 Christmas obtained a pyogeiiic body from the cultures in the 

 shape of a substance of the nature of a ferment, which set 

 up suppuration when introduced into the anterior chamber 

 of a rabbit's eye. E. Ullmann caused osteomyelitis by intra- 

 venous injection of dead cultures after a previous fracture. 



Streptococci. Emmerich and Hartmann succeeded in 

 isolating a streptococcus from the air, which, when inocu- 

 lated on rabbits, set up a typical erysipelas, and is there- 

 fore described as Streptococcus erysipelatis. A pure culti- 

 vation was first obtained by Fehleisen. Gelatine is not 

 liquefied. On the plate small colonies appear in the sub- 

 stance of the gelatine on the third or fourth day, and 

 gradually assume a brownish colour. In thrust cultures 

 the superficial growth is very scanty, but along the needle- 

 track very minute white globular colonie appear, forming a 

 white stripe. Small round isolated colonies develop upon 

 agar, resembling drops of dew. No growth takes place on 

 potatoes. 



According to Jordan, Erankel, and Von Eiselsberg, it is 

 identical with the Streptococcus pyogenes (see p. 201). 



Bacillus subtilis. This bacillus, also called the hay 

 bacillus, was described by Ehrenberg, and is most easily 

 obtained from an infusion of hay made by chopping up the 

 hay, pouring water on it in a flask, and bringing it once to the 

 boil. In this way all the other different micro-organisms are 

 easily killed, the hay bacillus alone suffering no impairment 

 of vitality. After two or three days a thick whitish pellicle 

 forms on the surface, and consists of a pure culture of the 

 Bacillus subtilis. The bacillus takes the form of very long, fine 

 thin rods, possessing marked power of movement by means 



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