170 BACTERIOLOGY 



rendered more easily visible if the blood be smeared on 

 a cover-glass with the edge of another, allowed to dry, 

 and stained with watery solution of methyl blue ; or, 

 according to Celli and Guarnieri, a good method is to dis- 

 solve the methyl blue in serum or ascitic fluid, and let it 

 run in from one edge upon a preparation of the blood, 

 which should not be previously dried. During the attack 



Amoeboid figure in a red corpuscle 



Remnant of the red corpuscle - (Rt^fc ^^ Other red corpuscles 



FIG. 66. PLASMODIUM OF MALARIA IN HUMAN* BLOOD, AT THE PERIOD OF AFYKEXIA. 



of fever the plasrnodia induce alterations in the corpuscle, 

 causing a conversion of the haemoglobin into melanine, so 

 that if the blood is examined after the attack the corpuscles 

 are found to be paler, -and show in their interior clumps 

 consisting of minute granules of black pigment (melancemia). 

 This formation of pigment advances so far that the blood 

 corpuscles are totally destroyed, and, according to Golgi, it 



Pigment 



Segmented plasmodium 



f*\ ^sy 



: Red corpuscles 



FIG. 67. PLASMODIUM OF MALARIA AT THE STAGE CORRESPONDING TO THK 

 TIME OF ONSET OF THE FEVER. 



goes on between the attacks. Multiplication of plasmodia 

 takes place by segmentation, and the new plasmodia at first 

 adhere to the edge of the blood-corpuscle, then become free 

 and subsequently penetrate again into other corpuscles 

 (fig. 67). According to Golgi, the process of segmentation 

 takes two days with some plasmodia, and with others three, 

 before the stage is reached at which the segmented portions 



