BACILLUS OF GLANDERS 219 



yellow or whitish round colonies on the plate as early as 

 the second day, while along a streak of inoculation made 

 on the same medium a shining coating appears in four to 

 five days. On the surface of blood-serum there are de- 

 veloped yellowish deposits which coalesce with one another 

 and form a slimy covering. The cultivation on potato is 

 particularly important : a deposit appears at incubation 

 temperature which is at first yellowish and transparent, 

 but which gradually increases and assumes a darker tint. 

 In about a week the colour changes to reddish-brown, and 

 finally passes into red. The bacilli readily take up the 

 aniline dyes, but discharge them again when Gram's method 

 is employed (fig. 84). 



a 



' 



FIG. 84. BACILLI OP GLANDERS IN HUMAN BLOOD. (After Jakscli.) 



The staining process of Loffler consists in colouring the 

 cover-glass preparations for five minutes in a hot alkaline 

 solution of gentian violet or fuchsine in aniline water con- 

 taining an addition of 1 per cent, of caustic soda solution. 

 Bleaching is done in 1 per cent, acetic acid coloured to a 

 wine yellow with a watery solution of tropseoline 00, in 

 which the preparations remain for an hour, and are then 

 rinsed in water. 



Kiihne treats the cover-glasses with hot carbolic fuchsine 

 or carbolic methyl blue, and decolorises in water contain- 

 ing two drops of hydrochloric acid per 100 grams. 



For staining glanders bacilli in sections, Kiihne has 

 devised the following method, which is reliable and easy in 



