220 BACTERIOLOGY 



use : The sections, having been stained with carbolic 

 fuchsine and soaked in water, are quickly decolorised in an 

 aqueous solution of hydrochloric acid (3 per cent.), well 

 rinsed in water, and then either dipped lightly into alcohol 

 or freed as far as possible from water by pressing them 

 with blotting-paper Aniline oil mixed with 20 per cent, 

 of oil of turpentine is now allowed to act on the sections in 

 a watch-glass for eight to ten minutes, during which they 

 remain adherent to the cover-glasses, and thence they are 

 transferred to oil of turpentine, xylol, and balsam. 



Noniewicz recommends that the sections should be 

 stained for a few minutes in alkaline methyl blue, rinsed 

 in water, and decolorised in a mixture of 3 parts of i per 

 cent, acetic acid, with 1 part of a ^ per cent, solution of 

 tropseoline 00 in water. Thin sections are only dipped in 

 quickly, but thick ones may remain in for two to five 

 seconds or longer. They are then washed in water, spread 

 out upon a slide, freed from moisture with blotting-paper, 

 dried in air, cleared with xylol, and mounted in Canada 

 balsam. The glanders bacilli appear black on a more or 

 less blue ground. By this method the discovery was made 

 that the characteristic bacilli appear in the acute form of 

 the disease, whereas in the chronic form they are sparser, 

 and round bodies, which stain intensely, appear in their 

 stead in large numbers. 



The glanders bacilli are also found in the interior of 

 dead cells. The vessels are free, and but few appear in the 

 blood. Transmission experiments made with pure cultures 

 have yielded successful results, the infection spreading most 

 from wounds in the skin. The cornmunicability of the 

 disease is considerable, and the infecting power of the virus 

 is not altered by drying for three months. 



Other microbes of pus. It need hardly be said that the 

 pus from wounds in other specific infectious processes con- 



