STAINING OF BACTERIA. 33 



VI. Carbolic Add Solutions. 



A. Ziehl-Nedsen. 



Fuchsin (powd.) .... 1 part. 



Alcohol 10 parts. 



5 per cent. sol. acid, carbolic . 100 parts. M. 

 Filter. The older the solution the better. 



B. Kuhne. 



Methylin blue . . . . 1.5 



Alcohol 10.0 



5 per cent. sol. ac. carbol. . . 100.0 

 Add the acid gradually. This solution loses strength with age. 



VII. Gram's Iodine Solution. 



Iodine 1 



Potass, iod 2 



Aquae destillat 300. M. 



VIII. Iyer's Mordant (for flagella). 

 Aq. sol. of tannin (20 per cent.) . 10 parts. 

 Aq. sol. ferri sulph. (5 per cent.) . 1 part. 

 Aquse decoc. of logwood (1-8) . 4 parts. M. 

 Keep in well-corked bottle. 



IX. Picro-carmine (Ranvier). 



Carmine 1 



Water 10 



Sol. ammon 3 



Sat. sol. picric acid . . . 200. M. 



X. Gabbet's Acid Blue (rapid stain). 

 Methylin blue . - . . . 2 

 25 per cent, sulphuric acid . . 100. M. 



XI. Alkaline Aniline Water Solutions. 

 Sodium hydrat. (1 per cent.) . . 1 

 Aniline oil water .... 100. M. 

 And add 



Fuchsin, or methyl-violet powd. . 4 

 Cork well. Filter before using. 

 3 



