STAINING AND MODIFICATIONS. 



37 



Dish III, water to rinse off the acid. The section can now be 

 placed under the microscope covered with cover-glass to see if 

 the intensity of the stain is sufficient or too great. A second 

 section is then taken, avoiding the errors, if any ; and having 

 reached this stage proceeded with as follows : 



Dish IV, alcohol, 2 to 3 seconds to remove the water in the 

 tissue. 



V. A few drops of oil of cZores, just long enough to clear the 

 specimen to make it transparent (so that an object placed under- 

 neath will shine through). 



VI. Remove excess with filter-paper. 

 VIL Mount in Canada balsam. 



FIG. 13. 



Section Microtome. 



