72 



ESSENTIALS OP BACTERIOLOGY. 



There is a slit along the top in which the tail can be fastened, 

 and thus the animal is secure and immobile. 



Intravenous Injections. Rabbits are very easily injected 

 through the veins. Mice are too small. 



The ear of the rabbit is usually taken. It is first washed with 

 1-2000 bichloride, which not only disinfects, but also makes the 

 vessels appear more distinct. The base of the ear is compressed 

 to swell the veins. Then a syringe, like the one used for the 

 injection of " tuberculine," a Koch syringe, which can be easily 

 sterilized, is filled with the desired amount of virus and slowly 

 injected into any one of the more prominent veins present. 

 (Fig. 43.) 



Intra-peritoneal Injection. This is used with Guinea-pigs 

 mostly. The abdominal wall' is pinched up through its entire 

 thickness, and the needle of the syringe thrust directly through, 

 so that it appears on the other side, then the fold let go, the 

 needle withdrawn just far enough so as to be within the cavity. 



Inoculation in the Eye. The anterior chamber and the cornea 

 are the two places used. The rabbit is fixed upon a board ; 

 the eyelids held apart and head held still by an assistant. A 

 small cut is made in the cornea, a few drops of cocaine having 



FIG. 43. 



Manner of making intravenous injections in the rabbit. 



