102 ESSENTIALS OF BACTERIOLOGY. 



-Loner's. (For cover-glass preparations.) 



1. Alkaline niethylin blue (Loffler's). 5 minutes. 



2. Acetic acid with a fe\v drops of tropseolin. 1 second. 



3. Washed in water. 



For Sections. Instead of tropaeolin acetic acid, the following 

 mixture is used : 



fy Oxalic acid 5 per cent. . . . gtt. j. 



Cone, sulphuric, acid gtt. ij. 



Aq. destill 31). M* 



The sections are kept in this 5 seconds. 

 Kuhne^s method. Covergfass. 



1. Warm carbol-blue 2 min. 



2. Decolorized in weak sol. of muriatic acid (10 parts to 500). 



3. Washed in water. 

 Sections of Tissue. 



1. Carbol-blue, $ hour. 



2. Decolorized in per cent, muriatic acid. 



3. Washed in distilled water. 



4. Dehydrated in alcohol 1 second. 



.">. Aniline oil with 6 gtts. of turpentine. 5 min. 



6. Turpentine, xylol, Canada balsam. 



If contrast stain, add 5 gtts. of safranin (Bismark-brown) to 

 turpentine, and use it after the xylol. 



Patlwgenesis. If horses, field mice, or guinea-pigs be inocu- 

 lated subcutaneously, with but a very small quantity of culture, 

 a local affection results, followed some time after by a general 

 disturbance ; ulcers form at the point of inoculation ; little 

 nodules, which then caseate, leaving scars and involving the 

 lymphatics ; metastatic abscesses then occur in the spleen and 

 lungs, and death arises from exhaustion. Cattle, pigs, and rab- 

 bits are not easily affected ; man is readily attacked. 



Manner of Infection. Ganders being a highly contagious dis- 

 ease, it requires but a slight wound to allow it to gain entrance. 



In horses the primary sore seems to be at the nasal mucous 

 membrane. In man it is usually on the fingers. Boiling water 

 or 1-10,000 sublimate solution will quickly destroy the virulence 

 of this bacillus. 



