126 ESSENTIALS OF BACTERIOLOGY. 



33 and 37 C., and the growth occurs very slowly and sparsely. 



In three days a very thin, almost invisible, moist yellowish 

 growth, seeming to be composed of little drops. 



Under low power small processes are seen shootin- out from 

 the smooth border. 



FIG. 



Gonococci in pus. 



It requires to be then transferred to fresh media, as it quickly 

 perishes. 



Staining. Colored easily with all ordinary aniline stains. 



Gram's method is not applicabk, this being one of its main 

 diagnostic features. 



The following method for coloring cover-glasses is recom- 

 mended by Neisser. 



The cover-glasses, with some of the urethral discharge smeared 

 upon them, are covered with a few drops of alcoholic solution of 

 eosin and heated for a few minutes over the flame. The excess 

 of the dye is removed with filter paper, then the cover-glass 

 placed in concentrated methylin blue (alcoholic solution) for 15 

 seconds, and rinsed in water. 



The gonococci are dark blue, the protoplasm of the cell pink, 

 and the nucleus a light blue, the gonococci lying in the proto- 

 plasm next to the nucleus. 



Other bacteria are similar to the gonococci in form ; they are 



