128 ESSENTIALS OF BACTERIOLOGY. 



at brood-heat. The colonies are as small white points, which 

 under low power appear brown and opaque. 



Agar Stroke Culture. Grayish-white growth, which after two 

 months is like a skin upon the surface. 



Staining. Takes Gram's method. 



Pathogenesis. None known. 



Micrococcus Subflavus. (Bunim.) 



Origin. In lochial discharges, in vagina and urethra of 

 healthy persons. 



Form. As gouococci. 



Properties. Not motile ; liquefy gelatine slowly ; a yellow- 

 brownish pigment. 



Growth. Grows slowly on all media, forming on gelatine, 

 after two weeks, a moist yellowish surface growth. 



Potato. Small half-moon-shaped colonies which, after three 

 weeks, become light-brown in color, and covering the surface as 

 a skin. 



Staining. Colored with Gram. 



Pathogenesis. Not acting upon the mucous membrane, but 

 when injected in cellular connective tissue, an abscess results 

 which contains myriads of diplococci. 



The gonococcus is distinguished from all these similar micro- 

 cocci by being found usually within the cell protoplasm. 



Secondly. Not stained with Gram-s method. 



Thirdly. Refusing to grow readily upon gelatine. 



All the similar bacteria being easily cultivated. 



These characteristics, taken in toto, form sufficient features for 

 its ready recognition, and as it is often a serious question to 

 decide, not so much because of the patient's health as because 

 of his character, we should be very careful not to pronounce a 

 verdict until we have tested the micro-organism as above. When 

 the germ so tested is found, the process can be called specific 

 without a doubt. 



Bacillus of Tetanus. (Nicolaier-Kitasato.) 



Origin. Nicolaier found this bacillus in the pus of a wound 

 in one who had died of tetanus, describing it in 1884. 



Kitasato has since then been able to isolate and cultivate this 

 germ. (1889.) 



