Micro-organisms of Maple Sap 



339 



NUMBER OF ORGANISMS IN SOUS MM'l.i. SAP 

 The results obtained with various samples of sour sap plated 

 at one or another time during the progress of the work are 

 given below in tabular form. Some of the material plated was 

 just beginning- to show signs of clouding while in other cases the 

 process was well advanced. In general the degree of decom- 

 position and the number of organisms found bore a direct re- 

 lation to each other. 



As may be seen from the table the count obtained on nutrient 

 agar varied from 320,000 to 141,420,000 per cc. Allowing an 

 average size of 1 micron by 3 microns the organisms represent 

 approximately one-third the volume of the most heavily infected 

 sap. 



TABLE 1. ORGANISMS PER CC. IN SOUR MAPLE SAP 



NO. 1 

 1 



2 



O 

 O 



4 



5 



6 



7 



8 



9 

 10 

 11 

 12 

 13 

 14 

 15 

 16 

 17 

 18 

 19 

 20 

 21 

 22 

 23 

 24 

 23 

 2G 



Nutrient 

 agar 



3,084,000 

 0,100,000 



21,300,000 



6,300,000 



1,272,000 



2,785,000 



320,000 



2,000,000 



843,000 



975,000 



8,276,000 



1,950,000 



6,500,000 



19,092,000 

 1,357,000 

 1,909,000 

 1,751,000 



10,000,000 



88,200,000 

 1,950,000 



14,500,000 

 8,850,000 

 7,300,000 

 2,150,000 

 5,540,000 

 7,500,000 



Synthetic 

 agar 



200,000 

 2,000 



13,000 



715, 000 



30,000 

 200,000 

 175,000 



20,000 



1.300 



500,000 



3,000 



254,500 



2,450,000 



10,000 



500 

 30,000 

 17,500 



No. 



27 

 28 

 29 

 30 

 31 

 32 

 33 

 34 

 35 

 36 

 37 

 38 

 39 

 40 

 41 

 42 

 43 

 44 

 45 

 46 

 47 

 48 

 49 

 50 

 51 

 52 



Nutrient 

 agar 



14,000,000 



5,667,000 



4,225,000 



19,500,000 



23,000,000 



9,750,000 



325,000 



4,875,000 



10,400,000 



43,875,00(1 



11,275.01)0 



56,250,000 



2,250,000 



1,625,000 



12,350,000 



3,262,500 



23,400,000 



29,250,000 



11,700,000 



9,480,000 



14,670,000 



6,500,000 



73,125,000 



65,000,000 



13,000,000 



141,420,000 



Synthetic 

 agar 



9,546,000 

 51,000 



15,000 



37.00(1 



10,500 



407.000 



60,000 



65.000 



646,300 



ST, 75(), Oilii 



100,000 



3. 040. 000 



•This series, 1-52, is entirely distinct from the similarly numbered 

 series later discussed on pages 351 to 365. 



